| 26.11.2020 19:45 | | Barrow | | AFC Wimbledon | | 0:0 aet, pen. 2:4 * |
| Barrow-in-Furness |
| 09.11.2020 19:45 | | Oxford City | | Northampton Town | | 2:1 (1:1) * |
| Marston |
| 08.11.2020 14:30 | | Eastbourne Borough | | Blackpool | | 0:3 (0:0) * |
| Eastbourne |
| 08.11.2020 12:45 | | Barnet | | Burton Albion | | 1:0 (1:0) * |
| London |
| 08.11.2020 12:45 | | Eastleigh | | Milton Keynes Dons | | 0:0 aet, pen. 3:4 * |
| Eastleigh |
| 08.11.2020 12:45 | | Hampton & Richmond Borough | | Oldham Athletic | | 2:3 (1:2) * |
| London |
| 08.11.2020 12:45 | | Havant & Waterlooville | | Cray Valley Paper Mills | | 1:0 (1:0) * |
| Havant |
| 08.11.2020 12:45 | | Hayes & Yeading United | | Carlisle United | | 2:2 aet (0:0, 0:0), pen. 3:4 * |
| London |
| 08.11.2020 12:45 | | Maldon & Tiptree | | Morecambe | | 0:1 (0:1) * |
| Maldon |
| 08.11.2020 12:45 | | Torquay United | | Crawley Town | | 5:6 aet (2:0, 3:3) * |
| Torquay |
| 08.11.2020 12:45 | | Wigan Athletic | | Chorley | | 2:3 aet (2:1, 2:2) * |
| Wigan |
| 08.11.2020 12:00 | | Scunthorpe United | | Solihull Moors | | 2:3 (1:1) * |
| Scunthorpe |
| 07.11.2020 17:30 | | United of Manchester | | Doncaster Rovers | | 1:5 (1:4) * |
| Manchester |
| 07.11.2020 15:00 | | Banbury United | | Canvey Island | | 1:2 (0:1) * |
| Banbury |
| 07.11.2020 15:00 | | Bolton Wanderers | | Crewe Alexandra | | 2:3 (1:1) * |
| Bolton |
| 07.11.2020 15:00 | | Boreham Wood | | Southend United | | 3:3 aet (1:0, 2:2), pen. 4:3 * |
| Borehamwood |
| 07.11.2020 15:00 | | Brackley Town | | Bishop's Stortford | | 3:3 aet (2:0, 3:3), pen. 3:2 * |
| Brackley |
| 07.11.2020 15:00 | | Bromley | | Yeovil Town | | 0:1 aet (0:0, 0:0) * |
| Bromley |
| 07.11.2020 15:00 | | Cambridge United | | Shrewsbury Town | | 0:2 (0:0) * |
| Cambridge |
| 07.11.2020 15:00 | | Charlton Athletic | | Plymouth Argyle | | 0:1 (0:0) * |
| London |
| 07.11.2020 15:00 | | Cheltenham Town | | South Shields | | 3:1 (1:1) * |
| Cheltenham |
| 07.11.2020 15:00 | | Colchester United | | Marine | | 1:1 aet (0:1, 1:1), pen. 3:5 * |
| Colchester |
| 07.11.2020 15:00 | | Dagenham & Redbridge | | Grimsby Town | | 3:1 (1:0) * |
| London |
| 07.11.2020 15:00 | | Gillingham | | Woking | | 3:2 (0:1) * |
| Gillingham |
| 07.11.2020 15:00 | | Hull City | | Fleetwood Town | | 2:0 (1:0) * |
| Kingston upon Hull |
| 07.11.2020 15:00 | | Ipswich Town | | Portsmouth | | 2:3 aet (1:2, 2:2) * |
| Ipswich |
| 07.11.2020 15:00 | | Leyton Orient | | Newport County | | 1:2 (1:1) * |
| London |
| 07.11.2020 15:00 | | Lincoln City | | Forest Green Rovers | | 6:2 (2:0) * |
| Lincoln |
| 07.11.2020 15:00 | | Oxford United | | Peterborough United | | 1:2 (1:1) * |
| Oxford |
| 07.11.2020 15:00 | | Port Vale | | King's Lynn Town | | 0:1 (0:0) * |
| Stoke-on-Trent |
| 07.11.2020 15:00 | | Rochdale | | Stockport County | | 1:2 (1:2) * |
| Rochdale |
| 07.11.2020 15:00 | | Salford City | | Hartlepool United | | 2:0 aet (0:0, 0:0) * |
| Salford |
| 07.11.2020 15:00 | | Stevenage | | Concord Rangers | | 2:2 aet (1:1, 1:1), pen. 5:4 * |
| Stevenage |
| 07.11.2020 15:00 | | Sunderland | | Mansfield Town | | 0:1 (0:0) * |
| Sunderland |
| 07.11.2020 15:00 | | Swindon Town | | Darlington | | 1:2 (1:1) * |
| Swindon |
| 07.11.2020 15:00 | | Tranmere Rovers | | Accrington Stanley | | 2:1 (2:1) * |
| Birkenhead |
| 07.11.2020 15:00 | | Walsall | | Bristol Rovers | | 1:2 (0:2) * |
| Walsall |
| 07.11.2020 13:00 | | Exeter City | | AFC Fylde | | 2:1 (2:1) * |
| Exeter |
| 07.11.2020 12:30 | | Tonbridge Angels | | Bradford City | | 0:7 (0:3) * |
| Tonbridge |
| 06.11.2020 19:45 | | Harrogate Town | | Skelmersdale United | | 4:1 (2:0) * |
| Harrogate |